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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
21/02/2014 |
Actualizado : |
02/09/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BERNAL, R.; MENDOZA, Y.; ORIHUELA, C. |
Afiliación : |
ROBERTO FRANCISCO BERNAL PIACENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; YAMANDU MENDOZA SPINA, Agronomists contracted for the Project Salto Grande Experiment Station, Uruguay; C. ORIHUELA, Agronomists contracted for the Project Salto Grande Experiment Station, Uruguay. |
Título : |
Alternatives to methyl bromide in Uruguay: Process to phase out this chemical. (Conference paper). |
Fecha de publicación : |
2005 |
Fuente / Imprenta : |
Acta Horticulturae, December 2005, N°698, p.33-38. Doi: https://doi.org/10.17660/actahortic.2005.698.3 |
Serie : |
(Acta Horticulturae; 698) |
ISBN : |
978-90-66052-18-5 |
ISSN : |
0567-7572 (print) // 2406-6168 (electronic) |
DOI : |
10.17660/actahortic.2005.698.3 |
Idioma : |
Inglés |
Notas : |
Article history: 31 December 2005. In: Acta Horticulturae (ISHS) 698: VI International Symposium on Chemical and non-Chemical Soil and Substrate Disinfestation - SD2004. Editor: A. Vanachter. Convener: E. Tjamos. |
Contenido : |
ABSTRACT.
Uruguay has 400 hectares of protected vegetable crops in the north of the country dedicated to early production. In this area, there are two zones where the nematode problems are very important since crops, such as tomatoes and peppers, almost have a ten-month of harvest period. In one zone, soils are heavy, while in other major places soils are sandy loam. In the heavy soil area, methyl bromide has already been phased out. The alternatives used in both locations are soil solarization alone or in combination with metham sodium or with buried chopped corn plant at the mature green corn stage. Solarization is done during thirty days with the greenhouse closed, and this technology is recommended from mid-December to the end of January to get the highest temperatures during the summer. Beneficial organisms like fluorescent Pseudomonas and Bacillus spp. reproduced satisfactorily in plots with the incorporation of organic amendments.
@ International Society for Horticultural Science. |
Palabras claves : |
Chemical products; Crop yield; Greenhouse product; Nematodes; Organic amendments; Soil disinfestation; Soil solarization. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02149naa a2200289 a 4500 001 1007926 005 2020-09-02 008 2005 bl uuuu u00u1 u #d 020 $a978-90-66052-18-5 022 $a0567-7572 (print) // 2406-6168 (electronic) 024 7 $a10.17660/actahortic.2005.698.3$2DOI 100 1 $aBERNAL, R. 245 $aAlternatives to methyl bromide in Uruguay$bProcess to phase out this chemical. (Conference paper).$h[electronic resource] 260 $c2005 490 $a(Acta Horticulturae; 698) 500 $aArticle history: 31 December 2005. In: Acta Horticulturae (ISHS) 698: VI International Symposium on Chemical and non-Chemical Soil and Substrate Disinfestation - SD2004. Editor: A. Vanachter. Convener: E. Tjamos. 520 $aABSTRACT. Uruguay has 400 hectares of protected vegetable crops in the north of the country dedicated to early production. In this area, there are two zones where the nematode problems are very important since crops, such as tomatoes and peppers, almost have a ten-month of harvest period. In one zone, soils are heavy, while in other major places soils are sandy loam. In the heavy soil area, methyl bromide has already been phased out. The alternatives used in both locations are soil solarization alone or in combination with metham sodium or with buried chopped corn plant at the mature green corn stage. Solarization is done during thirty days with the greenhouse closed, and this technology is recommended from mid-December to the end of January to get the highest temperatures during the summer. Beneficial organisms like fluorescent Pseudomonas and Bacillus spp. reproduced satisfactorily in plots with the incorporation of organic amendments. @ International Society for Horticultural Science. 653 $aChemical products 653 $aCrop yield 653 $aGreenhouse product 653 $aNematodes 653 $aOrganic amendments 653 $aSoil disinfestation 653 $aSoil solarization 700 1 $aMENDOZA, Y. 700 1 $aORIHUELA, C. 773 $tActa Horticulturae, December 2005, N°698, p.33-38. Doi: https://doi.org/10.17660/actahortic.2005.698.3
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
15/08/2022 |
Actualizado : |
01/12/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A. |
Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; DENISE D. GUERREIRO, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; OTÁVIO, K.S., Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; PEDRO C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; RICHARD NUÑEZ?OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARTINA CRIPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FÁBIO R. VASCONCELOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; MARIA JULIA B. BEZERRA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; RENATO F. SILVA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; LARITZA F. LIMA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; JOSÉ RICARDO FIGUEIREDO, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; IVAN C. BUSTAMANTE-FILHO, Laboratório de Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; ARLINDO A. MOURA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil. |
Título : |
How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?. |
Complemento del título : |
Volume 89, Issue 10, Pages 459 - 470October 2022 |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638 |
DOI : |
10.1002/mrd.23638 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. |
Contenido : |
Abstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. MenosAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols ... Presentar Todo |
Palabras claves : |
FOLLICLE; OVARY; OVINE; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; PROTEINS; REPRODUCTION. |
Asunto categoría : |
-- |
Marc : |
LEADER 03782naa a2200409 a 4500 001 1063525 005 2022-12-01 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1002/mrd.23638$2DOI 100 1 $aPASSOS, J.R.S. 245 $aHow in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?.$h[electronic resource] 260 $c2022 500 $aArticle history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. 520 $aAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. 653 $aFOLLICLE 653 $aOVARY 653 $aOVINE 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aPROTEINS 653 $aREPRODUCTION 700 1 $aGUERREIRO, D.D. 700 1 $aKAMILA S. OTÁVIO 700 1 $aDOS SANTOS-NETO, P.C. 700 1 $aSOUZA-NEVES, M. 700 1 $aCUADRO, F. 700 1 $aNUÑEZ?OLIVERA, R. 700 1 $aCRISPO, M. 700 1 $aVASCONCELOS, F.R. 700 1 $aBEZERRA, M.J.B. 700 1 $aSILVA, R.F. 700 1 $aLIMA, L.F. 700 1 $aFIGUEIREDO, J.R. 700 1 $aBUSTAMANTE-FILHO, I.C. 700 1 $aMENCHACA, A. 700 1 $aMOURA, A.A. 773 $tMolecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638
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